Mapping of seed coat colour in brassica juncea using TE-AFLP markets
Student name: Ms Shilpi Bhatia
Guide: Dr Anandita Singh
Year of completion: 2013
Host Organisation: The Energy and Resource Institute (TERI), New Delhi
Supervisor (Host Organisation): Dr S.B. Tripathi
Abstract: A Brassica juncea (2n=36) BC1 population of 100 individuals was developed by crossing two
cultivars of B.juncea viz. Indian variety Pusa Bold and a canola quality variety Heera. The two
lines chosen are morphologically and genetically diverse and contain several valuable
agronomic quantitative and qualitative traits. TE-AFLP fingerprints were generated with the use
of three restriction enzymes EcoRI, MseI and Pst1. Using 3 primer combinations, a total of 35
parental polymorphic bands were detected. Phenotypic data for several important traits was also
assessed and correlation between these characters was calculated with two different method;
Pearson’s method and Single marker (ANOVA) analysis. Linkage analysis was performed using
the program JoinMap ver.4.0 with a final BC1 population of 56 individuals and 36 loci. A
significant linkage map could not be generated due to a low poppulation size and less number of
polymorphic markers employed. However, a significant correlation was found between 1000
seed weight and pod length as well as pod width. Several loci were also found to have a
significant correlation with these traits.
Keywords: TE-AFLP, Brassica juncea, linkage map, canola quality, erucic acid, glucosinolates,
correlation
