Proteomic analysis of exogenous sodium nitroprusside induced genes under dehydration in rice
Student name: Ms Garima Vishnani
Guide: Dr Deepti Gupta
Year of completion: 2013
Host Organisation: TERI University
Abstract: Abiotic stresses in plants such as dehydration, salinity, high and low temperature,
water logging have been a major threat to agriculture in the past few years.
Therefore a study of plant defense mechanism against these stresses has become a
challenging task in the field of plant biotechnology. Nitric oxide is involved in the
stress signaling pathway in plants. It induces various stress inducible genes during
dehydration stress in plants. In the following study, the effect of 100μM Sodium
Nitroprusside on alleviation of dehydration stress in rice (Oryza sativa) plants has
been investigated through various physiological assays and 2-Dimensional gel
electrophoresis. The physiological and enzyme assays indicate that Sodium
Nitroprusside treated plants after 72 hours and 96 hours of treatment show
significant alleviation of dehydration stress in comparison to dehydrated plants and
have thus been chosen for further proteomic studies. 1-DE and 2-DE analysis
showed a presence of many new and differentially expressed proteins in the
treatment plants. These bands were further analyzed by mass spectrometry and
searched against MASCOT database for their identification.
Key words: dehydration stress, rice, nitric oxide, 2-DE, mass spectrometry
