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Effect of various growth regulators on in vitro shoot multiplication of solanum tuberosum

Student name: Ms Anjali Bala
Guide: Dr Anandita Singh
Year of completion: 2010
Host Organisation: The Energy and Resources Institute (TERI) Micropropagation Technology Park (MTP), Gual Pahari, Harya
Supervisor (Host Organisation): Dr Sanjay Saxena
Abstract: The in vitro growth of several genotypes of Solanum tuberosum (Chipsona 3, Himalini, Shailja, Sadabahar, Lauvkar) on media containing various plant growth regulators has been studied with a view to improve various growth parameters like multiplication fold, shoot quality etc. and the overall survival of the plantlets. Different concentrations of the phloroglucinol (0, 0.4, 0.6, 0.8, 1.0 and 1.2 mM) was tested alone and then in combination with three different concentrations of sucrose (0.1, 0.15 and 0.2M). While the phloroglucinol alone was able to improve the multiplication fold and the shoot length, there was significant phloroglucinol × sucrose interaction. Where phloroglucinol was used alone, highest multiplication fold and shoot length was observed at 1.2mM concentration for all the genotypes studied, whereas in combination with sucrose, highest multiplication fold and shoot length was observed at either 1.0 or 1.2mM phloroglucinol with any one of the three sucrose concentrations. The response was variety dependent. There was no change in the response of any genotypes due to change in sucrose concentrations alone. Different concentrations of calcium nitrate (0, 5, 10, 15 and 20mM) was also used to study the effect of calcium on in vitro growth. While at lower concentrations of calcium nitrate, there was no change in any of the growth parameters studied, there was an increase in the multiplication fold and length of the shoots at higher concentrations like 15 or 20mM. In vitro growth was also studied under three different light conditions (increased photoperiod, increased intensity and both). While there was no change in multiplication fold, there was a decrease in length of the shoots which led to increase in their thickness.

Keywords: Solanum tuberosum, in vitro shoot multiplication, phloroglucinol, calcium nitrate, light intensity and photoperiod