ANNOUNCEMENTS
Standardization of DNA isolation ensures substantial implementation of procedures required to eliminate contamination and provide good yield with an adequate level of purity. It helps to validate the accuracy of the obtained outcome by ensuring a competent and significant degree of isolated DNA, which facilitates the comparison of results across different laboratories, allowing for collaborative research and meta-analyses. DNA isolation of a perennial medicinal plant like Desmodium gangeticum becomes challenging due to its rich secondary metabolite content, complex tissue composition, and high polyphenolic content. Overcoming these challenges necessitates the development of optimized DNA isolation protocols tailored to Desmodium gangeticum, incorporating techniques to mitigate the effects of secondary metabolites and ensure the extraction of high-quality DNA for downstream genetic and genomic analyses. Progressively, the construction of a genotyping-by-sequencing (GBS) library using isolated DNA from Desmodium gangeticum involves a series of meticulous steps developed to score the plant's unique genotypic constitution. The current study aims to streamline the DNA isolation process for Desmodium gangeticum by standardizing protocols using both commercial kits and the CTAB method, while also comparing different isolation methods based on key parameters. This comparative analysis assessed DNA concentration, purity, and DNA digestion analysis to determine the most efficient approach. As a result, an optimized DNA isolation protocol was developed. Using this protocol, a genotyping-by-sequencing (GBS) library was prepared with the isolated DNA of Desmodium gangeticum, enabling thorough genetic analysis and facilitating the study of genetic diversity, population structure, and trait variation within this plant species.
Keywords: Desmodium gangeticum, DNA Isolation, Genotyping-by-Sequencing, CTAB.
