ANNOUNCEMENTS
Hepatocellular carcinoma (HCC), also referred to as primary liver cancer, is one of the main causes of death for those with chronic liver disease. The frequency and etiological factors of HCC vary greatly throughout nations, which is indicative of regional variations. Hepatocellular carcinoma (HCC) is known to be associated with several risk factors, including alcohol misuse, non-alcoholic fatty liver disease, chronic hepatitis B and C infections, and aflatoxin effects. Diabetes and smoking are regarded as risk factors.(Rao & Kulkarni, 2023). Cirrhosis is strongly associated with HCC development.(Desai et al., 2019).
The modern non-classical MHC class I protein known as human leukocyte antigen-G (HLA-G) is distinct from classical HLA class I (A, B, and C) molecules in that it has a limited tissue distribution and minimal polymorphism. The HLA-G gene, which codes for the heavy chain of the molecule and is homologous to other HLA class I (Ia) genes, is found on chromosome 6 in the 6p21.3 area. It is composed of 7 introns and 8 exons. Exons 5 and 6, correspondingly, encode the transmembrane and cytoplasmic domain. Exon 7 is removed from mature mRNA and exon 8 is left untranslated due to the stop codon in exon 6 (Carosella et al., 2008). The HLA-G gene has variations in its 5′ upstream regulatory region(5’URR) and the 3′ untranslated region(3’UTR) including single nucleotide polymorphisms (SNPs) and insertion-deletion (INDELs) (Castelli et al., 2021). In this project, we have summarized HLA-G expression in Hepatocellular carcinoma (HCC) with a particular focus on the molecular mechanism involved in the interference with the immune effectors. In addition, a 3’UTR clone has been generated for use in HCC to check the miRNA targeting the HLA-G gene.
Objectives-
• Identification of microRNA targeting HLA-G UTR through bioinformatics analysis.
• Cloning of HLA-G UTR in psiCHEK-2 Reporter vector.
• To check the effect of microRNA targeting HLA-G UTR.
