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Investigation of biochemical mechanism for anaerobic germination of deep water rice

Student name: Ms Sandhya Yadav
Guide: Dr Shashi Bhushan Tripathi
Year of completion: 2018
Host Organisation: National Institute of Plant Genome Research (NIPGR), New Delhi
Supervisor (Host Organisation): Dr Jagadis Gupta Kapuganti
Abstract: Almost about 50% of the world population is dependent on rice (Oryza sativa). Also, the population of the world is going to rise in the coming years. Therefore, the yield of rice is to be increased by at least 30-40% over the time period. This significant increase in the yield of rice would lessen the pressure on the world’s food production. Also, germination of seed under very low oxygen condition is becoming an area of active research. NO is a gas, which is often found in radical form. NO has many roles to play in plants ranging from the signalling molecule during stress to germination of seeds, development of seedling, pathogen protection, this signalling molecule is used throughout the life of the plant. Just like ROS, plants produce NO during their normal growth and the production of NO often increases when the stress in plant increases. Also according to some reports there is a significant increase in nitric oxide levels in hypoxia as compared to normoxic conditions. There are number of methods available to measure NO levels in plants, for e.g.:- Griess diazotization reaction and then the detection of nitrite available through spectrophotometer. Nitrate reductase assay can also be helpful in determining the amount of nitrite in plant. DAF staining can also be used to detect nitrite content available in plants as it detects levels of low concentrations of NO.