Mapping of quantitative trait loci (QTLs) linked to virus resistance in Jatropha
Student name: Mr Muhammad Ibrahim Isiaka
Guide: Dr Anandita Singh
Year of completion: 2016
Host Organisation: The Energy and Resource Institute (TERI), New Delhi
Supervisor (Host Organisation): Dr Shashi Bhushan Tripathi
Abstract: Present efforts to massive economical cultivation of Jatropha curcas L. for biodiesel are impeded by a deadly virus disease. The causative virus, Jatropha mosaic virus (JMV), belongs to the genus Begomovirus of the family Geminiviridae. The virus causes leaf curl which subsequently results in shrunken leaf size, retarded growth and absence of flowering and fruiting. As a major step towards development of virus resistance breeding variety, a validation of AFLP and SSRs markers found to be highly correlated to virus resistance in a BC1 population generated from Jatropha curcas (virus susceptible) and Jatropha integerrima (virus resistance) was carried out. Among the 11 linkage groups (LG) already developed from BC1 population, LG3 and LG11 had 2 and 5 markers, respectively, which were found to be significantly associated with virus resistance. Resistance to the virus with these markers was evaluated using an advanced breeding population derived from random crosses between BC1 population plants under field condition in Eluru, Andhra Pradesh. Of the 5 AFLP markers, E-AACxM-CAT07 showed significant correlations with the virus phenotypic data. Graphical Genotypes (GGT) maps of LG3 and LG11 constructed using AFLP and SSRs genotyping and virus phenotypic data showed that about 55% and 46% of plants carry the homozygous virus resistance gene locus, respectively. The markers showing significant association will be useful in developing virus resistance Jatropha varieties.
Keywords: Jatropha mosaic virus, AFLP, SSRs, virus resistance, Graphical genotype
