Studying the effect of exogenous chemical compounds and molecular cloning of stress responsive OsMADS23 in Oryza sativa under dehydration stress
Student name: Ms Hiru Ranabhatt
Guide: Dr Anandita Singh
Year of completion: 2015
Host Organisation: National Institute of Plant Genome Research (NIPGR), New Delhi
Supervisor (Host Organisation): Dr Niranjan Chakraborty
Abstract: Rice is the most important staple crop and its productivity is highly influenced by water
levels. Dehydration stress is the major cause influencing rice production worldwide.
Oxidative damage is often induced by abiotic stresses in plants. The present study is the
first ever studied investigation on dehydration stress-induced morphological,
physiological and biochemical changes in rice seedlings. The study focuses on the effect
of foliar application of 20 Mm SNP, 4 Mm CaCl2 and 50 ppm GA on leaf physiology and
biochemistry. Differentially expressed proteins have been tested for their stress
alleviating ability. Physiological assays showed that all the treatments were effective in
alleviation of stress. Furthermore, GA and CaCl2 were found to be most effective in
maintaining the relative water content of the seedlings. SDS page of the proteins
isolated from the three treatments also showed differential protein expression, implying
that these cause perturbations in different signaling pathways. OsMADS23, previously
identified stress responsive candidate under CaCl2 signallome was isolated and
sequence characterized by phylogenetic analysis along with other 70 rice MADS-box
gene sequences. In summary, our result reveals that foliar application of exogenous
chemical compounds in an appropriate concentration, might account for the alleviating
effect of dehydration stress on rice plants.
Key words: nitric oxide, gibberellic acid, calcium chloride, MALDI-TOF, MADS-box.
