Genetic diversity analysis of Ocimum accessions using RAPD markers
Student name: Ms Garima Pal
Guide: Dr Sonika Gupta
Year of completion: 2015
Host Organisation: National Bureau of Plant Genetic Resources (NBPGR), Pusa
Supervisor (Host Organisation): Dr Ambika B. Gaikwad
Abstract: Ocimum belongs to the family lamiaceae and consists of about 160 species. Genetic diversity of
Ocimum was analysed among forty-five accessions using RAPD markers. In total, 69 primers
were screened out of which only 31 primers were further used in the study. 95.6% polymorphism
was detected in the samples.433 markers were identified out of which 414 markers were
polymorphic and 19 were monomorphic. Maximum numbers of markers were amplified with the
primer OPZ-13. The Average heterozygosity detected was 0.20. Resolving power ranged from
1.46-7.02 with OPZ-19 having highest resolving power. A UPGMA based dendrogram was
constructed with the Jaccard’s similarity coefficient which ranges from 0.1 to 1 with highest
similarity between the accessions 5 and 3, 2 and 3. Three major clusters were revealed by
UPGMA dendrogram. Cluster 1 consists of the accessions belonging from north western states.
Cluster 2 consists of the accessions from western hills and Gangetic plains. Cluster 3 consists of
the accessions from the eastern region. All the accessions were differentiated from each other
indicating the utility of RAPD markers in molecular characterization of Ocimum Study of
genetic diversity along with phenotypic and biochemical chacterization would help in identifying
the genotypes which are useful in industries, medicines, food etc.
Keywords: Ocimum, Genetic diversity, RAPD markers, UPGMA, Jaccard’s similarity
coefficient.
