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Announcement
Announcement
Deciphering bacterial non-coding RNAs of functional relevance from biofilm-forming bacteria

Student name: Ms Ankita Srivastava
Guide: Prof. Anandita Singh
Year of completion: 2022
Host Organisation: TERI-Deakin Nanobiotechnology Centre (TDNBC), Gual pahari, Gurugram
Supervisor (Host Organisation): Dr Mandira Kochar
Abstract:

Bacterial non-coding RNAs are implicated in numerous biological processes. These include, cellular and nucleotide metabolic cycles, amino acid metabolism, development of nutrient and pathogen stress response across many bacterial species. Here, we characterize non-coding RNAs of Priestia filamentosa. This bacteria is a gram-positive, rod shaped endospore forming endophytic bacteria, earlier known as Bacillus filamentosus. We recorded 168 non-coding RNAs from Priestia filamentosa RNA sequencing information and analysed their putative quality targets by means of IntaRNA web server tool. In-silico examination and different channels applied like grouping length upto 500bp, PresRAT sRNA score assurance, Rfam homologous search, secondary structure expectation, NCBI Blast Impact, domain search, proteins/enzymes included, we shortlisted four non-coding RNAs which assumes a larger part in utilitarian plant-microorganism interaction (for agricultural significance), sporulation, biofilm development, and biofertilizers. In future, integration of in-silico pipelines will facilitate evolving deeper insights on non-coding RNAs of Priestia filamentosa in mediating environmental stress response and biofertilizers. Hence, identification of bacterial ncRNAs and putative targets has turned into a significant piece of RNA science and practical importance in agricultural biome.

Keywords: Biofilm, Non-coding RNAs, putative target predictions (IntaRNA), Biofertilizers, Priestia filamentosa.