ANNOUNCEMENTS
Bamboo, known to be the fastest-growing crop in the world, is gradually taking over as the future timber. Many new techniques have been developed in recent years to suit the demands of this green treasure. The present study is based on the axillary bud break or in vitro shoot induction and multiplication of Bambusa vulgaris (var. green) nodal segments collected from field-grown culms across various genotypes using different cytokinins (BAP, Kn, TDZ, ADE) and concentrations to determine the most suitable media for maximal growth. In shoot induction, Adenine (ADE) and Kinetin (Kn) containing MS medium was shown to be the best for in vitro propagation. When BAP was supplemented at various concentrations, it was discovered that 5.0 mg/l in the initial sub-culturing MS medium resulted in the greatest number of shoots, whereas 3.0 mg/l BAP resulted in the greatest shoot length elongation in two genotypes after two weeks of incubation. An approach is also described with slight modifications to prior findings, for extraction of genomic DNA from both the mother plant and the in vitro propagated plants. A quantitative analysis of the isolated DNA using spectrophotometry was also performed to ensure the purity levels of the extracted DNA.
Key words: Micropropagation, BAP, Kn, TDZ, ADE.
