Development and validation of markers associated with seed coat color in Brassica juncea
Student name: Ms Utsa Rathi
Guide: Dr Anandita Singh
Year of completion: 2014
Host Organisation: The Energy and Resources Institute (TERI)
Supervisor (Host Organisation): Dr S.B. Tripathi
Abstract: A Brassica juncea (2n=36) F3 population was generated by selfing of F2
individuals derived from the cross (Pusa bold x Heera). Further, bulk segregant
analysis was conducted using 10 three-endonuclease AFLP primer combinations
on different bulks for seed coat color. To validate the previously published
markers linked to seed coat color in the present genetic background, the F3
population was genotyped with five co-dominant markers which included 3 SSR
and 1 SCAR markers selected from published literature (Negi et al. 2000;
Padmaja et al. 2005). The genotype data was used to map the genetic locus
controlling seed coat color. Three markers, namely, Na10A08, Ra2A11 and
SCAR08 grouped together with seed coat color locus at a high LOD score value
of 4. Maps created using both Haldane’s and Kosambi’s mapping functions
produced same marker order. However, as expected, the Kosambi map length
was slightly smaller than that obtained using Haldane’s mapping function.
Significant correlations were observed among the seed and pod traits such as
100 seed weight, pod length and pod width.
Keywords: Brassica juncea, canola quality, AFLP, TE-AFLP, linkage map, SSR,
SCAR, Bulk segregant analysis, correlation
