Characterization of dehydration stress responsive plasma membrane protein, CaDREPP1, in Cicer arietinum L.
Student name: Ms Sambhavana Chauhan
Guide: Dr Anandita Singh
Year of completion: 2015
Host Organisation: National Institute of Plant Genome Research (NIPGR), New Delhi
Supervisor (Host Organisation): Dr Niranjan Chakraborty
Abstract: Dehydration is the most critical and widespread abiotic stress endured by plants that affect water potential gradients, membrane integrity, and intracellular protein level by perturbing cellular homeostasis. This negatively influences plant development and productivity. The plasma membrane (PM) acts as a barrier between the cell and it’s environment in all living cells and is one of the most composite and differentiated membrane. In the present study, the deletion variants of dehydration-responsive plasma membrane protein, CaDREPP1, were cloned in expression vector and transiently expressed in the epidermal cells of onion peel. The localization pattern of 18ΔCaDREPP1 helped in understanding the importance of myristoylation in the second glycine position in the PM localization of the protein. PM protein expression profile of chickpea was characterised by 1-DE analysis. Phenotypic screening of CaDREPP1 mutant lines was carried out and subsequently compared with the wild-type and overexpression lines. Phenotype analysis revealed that mutants did not vary in their germination pattern as compared to the Col-0 wild-type seedlings on growing seeds on normal MS plates after stratification. However when non-stratified seeds were directly plated on experimental plates (supplemented with MV, H2O2, NaCl, ABA) the overexpression lines showed least germination frequency whereas the mutant lines (loss of function) performed better than the wild type. The PM protein expression profile of 72 hrs stressed chickpea seedlings was characterized by 1-DE analysis and 36 non-redundant proteins were identified including already reported plasma membrane proteins, like PATL2 (transporter protein), lipocalin (abiotic stress responsive protein) etc.
Key words: Abiotoic stress, Cellular homeostasis, Organellar proteome, Legume crop, Plasma membrane, PM proteome
