In silico analysis of chloroplast genome sequences of higher plants for identification of putative DNA barcoding regions
Student name: Ms Arpita Khurana
Guide: Dr Pallavi Somvanshi
Year of completion: 2014
Host Organisation: Gargi College, University of Delhi
Supervisor (Host Organisation): Dr Aparajita Mohanty
Abstract: Plant DNA Barcoding is an important technique to identify different plant/taxa and interpret the
intertaxa variations which can be used to separate the specimens by a distance called Barcoding
Gap. In the study seven intergenic regions of chloroplast genomes of six angiosperm families
have been analyzed .Since chloroplast genomes are structurally conserved, their intergenic
regions can be analyzed to search for putative barcodes therefore, the result of the present
investigation shows intraspecific, interspecific and intergenic variations within the family in the
seven intergenic regions. The nucleotide variations included insertions, deletions, and
substitutions. The regions flanking the variable regions were searched for the conserved regions.
These conserved regions were used to design primers that are predicted to amplify the putative
DNA barcode regions. Thus primers are expected to be universal within the members of the
angiosperm families studied.
Keywords: DNA Barcoding, intraspecific variation, interspecific variation, Universal Primer,
intergenic region.
